In response to the critical challenges posed by waste management and biomass production in long-duration space missions, such as those on the International Space Station (ISS) and future lunar bases or Gateway missions, this project proposed the development and demonstration of an innovative closed-loop bioreactor system utilizing insects. Traditional waste disposal methods in space, which often involve storage or return to Earth, contribute to orbital debris and increased logistical demands. By leveraging insect farming within a bioreactor, this project aimed to facilitate the enhancement of sustainability and reduction of operational costs associated with waste management and life support technologies in space environments.