This project concerns the proof of concept, design and construction of the first micro-incubator to enable biologists to optimise cell and tissue culture conditions and to get as close as possible to real-life physiological and pathological conditions. In the past, there has not been a mobile and autonomous biomedical device allowing both incubation and observation under multiple conditions in real time. This development therefore concerns a miniaturised incubator containing all the functionalities to be easily set up and allowing great transport mobility, the maintenance of culture conditions by perfusion of fluids and the observation at any time and without physical contact of the samples it contains.